Are the buses in Macau run everyday including Saturday and Sunday from 7AM to Midnight?

There are many public buses operating in the city everyday. May I know which bus you refer to? Or you want to know the timetable of the bus operating between Venetian and Seac Pai Van Park?
From your hotel, you can take bus no.26A at Hongshulin Bus Stop to Seac Pai Van Park Bus Stop directly. It works during 06:30 to 01:00 from Heisha Beach Bus Stop to North Shalitou Street Bus Stop, while 06:00 to 00:30 from North Shalitou Street Bus Stop to Heisha Beach Bus Stop with the interval of about 10 minutes. The ticket fare may be around MOP$3.6/person.